ChIP-Grade Protein G Magnetic Beads from Cell Signaling Technology

Product Specs
ItemChIP-Grade Protein G Magnetic Beads
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9006S
Quantity1 ml
ApplicationsChIP
Research AreaRelated Products
TypeResins, Magnetic Beads
ModeAntibody Purification

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Mitochondrial permeability transition regulator, cyclophilin D, is transcriptionally activated by C/EBP during adipogenesisJournal of Biological ChemistryDecember 1, 2023Chen Yu, Rubens Sautchuk, John Martinez, Roman A. Eliseevcontrol nonimmune IgG. Immunocomplexes were then purified with protein G magnetic beads (Cell Signaling, 9006), washed, and resuspended in 2x Laemmli buffer. Western blot analysis was performed as described above

(2) S1PR1 regulates ovarian cancer cell senescence through the PDK1-LATS1/2-YAP pathwayOncogeneNovember 17, 2023Yi-Ping Tao, Heng-Yan Zhu, Qian-Yuan Shi, Cai-Xia Wang, Yu-Xin Hua, Han-Yin Hu, Qi-Yin Zhou, Zi-Lu Zhou, Ying Sun, Xiao-Min Wang, et al.the chromatin extract was set aside for input. Antibody-protein-DNA complexes were bound to 30 μL of ChIP-grade protein G magnetic beads (Cell Signaling Technology) for 2 hours at 4 °C. The beads were washed 3 times with low-salt wash buffer

(3) The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activityVeterinary ResearchNovember 15, 2023Min Zhang, Lilei Lv, Huaye Luo, Hongming Cai, Lingxue Yu, Yifeng Jiang, Fei Gao, Wu Tong, Liwei Li, Guoxin Li, et al.CST) was added to the 20 million cell lysates along with ChIP-Grade Protein G Magnetic Beads (cat#: 9006, CST). The mixture was incubated overnight at 4 °C. On the second day, the beads were washed twice using

(4) Epigenetic regulation of beta-endorphin synthesis in hypothalamic arcuate nucleus neurons modulates neuropathic pain in a rodent pain modelNature CommunicationsNovember 9, 2023Yu Tao, Yuan Zhang, Xiaohong Jin, Nan Hua, Hong Liu, Renfei Qi, Zitong Huang, Yufang Sun, Dongsheng Jiang, Terrance P. Snutch, et al.chromatin. The supernatant incubated with IgG (Cell Signaling Technology) was used as a negative control. ChIP-grade protein G magnetic beads (Cell Signaling Technology) were used to capture protein-chromatin complexes. After washing, the protein-chromatin

(5) Wnt activation disturbs cell competition and causes diffuse invasion of transformed cells through NF-κB-MMP21 pathwayNature CommunicationsNovember 3, 2023Kazuki Nakai, Hancheng Lin, Shotaro Yamano, Shinya Tanaka, Sho Kitamoto, Hitoshi Saitoh, Kenta Sakuma, Junpei Kurauchi, Eilma Akter, Masamitsu Konno, et al.control (Cell signaling) overnight at 4 °C. The immunocomplexes were then rotationally incubated with ChIP-Grade Protein G Magnetic Beads for 2 h at 4 °C. ChIP DNA was eluted in ChIP elution buffer (IP sample) and subjected to q-PCR analyses

(6) Single-base editing in IGF2 improves meat production and intramuscular fat deposition in Liang Guang Small Spotted pigsJournal of Animal Science and BiotechnologyNovember 2, 2023Tianqi Duo, Xiaohong Liu, Delin Mo, Yu Bian, Shufang Cai, Min Wang, Ruiqiang Li, Qi Zhu, Xian Tong, Ziyun Liang, et al.200–400 bp. Chromatin extract was conjugated with G-protein magnetic beads (Cell Signaling Technology 9006, Boston, Massachusetts, USA), 10 μg ZBED6 antibody was added to control and experimental groups, the

(7) The E301R protein of African swine fever virus functions as a sliding clamp involved in viral genome replicationmBioOctober 31, 2023Su Li, Hailiang Ge, Yanhua Li, Kehui Zhang, Shaoxiong Yu, Hongwei Cao, Yanjin Wang, Hao Deng, Jiaqi Li, Jingwen Dai, et al.antibodies for 4 hours at 4°C and then incubated with the protein G magnetic beads (catalog no. 9003S#9006, Cell Signaling Technology) for 2 hours at 4°C. The beads were washed three times with wash buffer A

(8) HuR-mediated nucleocytoplasmic translocation of HOTAIR relieves its inhibition of osteogenic differentiation and promotes bone formationBone ResearchOctober 23, 2023Yuheng Li, Weijia Sun, Jianwei Li, Ruikai Du, Wenjuan Xing, Xinxin Yuan, Guohui Zhong, Dingsheng Zhao, Zizhong Liu, Xiaoyan Jin, et al.Cell Signaling Technology, Cat. No. 3900, monoclonal) overnight at 4 °C, followed by incubation at 4 °C with ChIP-Grade Protein G Magnetic Beads for 2 h. After washing, the eluted DNA was purified and assessed by qRT‒PCR to measure the amount of

(9) RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering EffectCellsAugust 3, 2023Wu J, Wang X, Zhang M, Mathews P, Kang Y.sonicated, and the chromatin solution was precleared with 30 μL of ChIP-grade protein G magnetic beads (#9006; Cell Signaling Technology, Danvers, MA, USA). The soluble fraction was collected, and the chromatin

(10) Longevity-Associated Transcription Factor ATF7 Promotes Healthspan by Suppressing Cellular Senescence and Systematic InflammationAging and diseaseAugust 1, 2023Yaqun Huang, Ming-Xia Ge, Yu-Hong Li, Jing-Lin Li, Qin Yu, Fu-Hui Xiao, Hong-Shun Ao, Li-Qin Yang, Ji Li, Yonghan He, et al.used as a negative control. The immunocomplexes were washed using ChIP-Grade Protein G Magnetic Beads (9006, Cell Signaling Technology, USA) and incubated at 65 °C overnight following the addition of 5 M NaCl

(11) The uncharacterized transcript KIAA0930 confers a cachexic phenotype on cancer cellsOncotargetJuly 20, 2023Yamakawa T, Zhang G, Najjar LB, Li C, Itakura K.Burlington, MA, USA) overnight at 4°C with gentle shaking. Thirty microliters of Protein G Magnetic Beads (#9006; Cell Signaling Technology) were then added, and the mixture was incubated for an additional 2 hours

(12) Ovol1/2 loss‐induced epidermal defects elicit skin immune activation and alter global metabolismEMBO reportsJuly 5, 2023Morgan Dragan, Zeyu Chen, Yumei Li, Johnny Le, Peng Sun, Daniel Haensel, Suhas Sureshchandra, Anh Pham, Eddie Lu, Katherine Thanh Pham, et al.rabbit anti‐Ovol1 antibody (Dai et al, 1998) and SimpleChIP® Enzymatic Chromatin IP Kit Magnetic Beads (9006, Cell Signaling) according to the manufacturer's instructions. DNA was then purified and NexteraPE adapters

(13) MiR-431 attenuates synaptic plasticity and memory deficits in APPswe/PS1dE9 miceJCI insightJune 22, 2023Ge J, Xue Z, Shu S, Yu L, Qin R, Tao W, Liu P, Dong X, Lan Z, Bao X, et al.rabbit IgG (Cell Signaling Technology, catalog 2729) on a rotary table overnight at 4°C. Then 30 μL of ChIP-Grade Protein G Magnetic Beads were added and incubated for 2 hours. DNA was eluted from antibody/Protein G Magnetic Beads, and the

(14) METTL16 promotes glycolytic metabolism reprogramming and colorectal cancer progressionJournal of experimental & clinical cancer research : CRJune 20, 2023Wei W, Zhang ZY, Shi B, Cai Y, Zhang HS, Sun CL, Fei YF, Zhong W, Zhang S, Wang C, et al.anti-IgG antibody (#2729, CST) at 4 °C overnight. The DNA-protein complex was incubated with 30 µl ChIP-grade Protein G magnetic beads for 2 h at 4 °C. After elution from the beads, the immunoprecipitates were de-crosslinked at 65 °C for

(15) Targeting KAT2A inhibits inflammatory macrophage activation and rheumatoid arthritis through epigenetic and metabolic reprogrammingMedComm (2020)June 11, 2023Yunkai Zhang, Ying Gao, Yingying Ding, Yuyu Jiang, Huiying Chen, Zhenzhen Zhan, Xingguang LiuSigma–Aldrich. Chicken Type II Collagen (20088) was from Chondrex. ChIP‐grade protein G magnetic beads (9006) and cell lysis buffer (9803) were from Cell Signaling Technology (CST). Nigericin (tlrl‐nig), ATP (tlrl‐atpl),

(16) Protocol to isolate RBP-mRNA complexes using RNA-CLIP and examine target mRNAsSTAR ProtocolsJune 1, 2023Valerie Blanc, Elizabeth A. Molitor, Nicholas O. Davidson,Thermo Scientific,N/A Peristaltic pump,Gilson,N/A ChIP-Grade Protein G Magnetic Beads,Cell Signaling,Cat#9006 Yeast tRNA,Thermo Fisher Scientific,Cat#AM7119 4-12% Bis-Tris,Invitrogen,Cat#NP0321BOX 10 mM dNTP

(17) Structural basis of CST-Polα/Primase recruitment and regulation by POT1 at telomeresbioRxivMay 27, 2023Sarah W. Cai, Hiroyuki Takai, Thomas Walz, Titia de LangeSamples were nutated at 4°C for 4 hours. 20 μL of Protein G magnetic beads slurry (Cell Signaling, 9006), preincubated with 5% Bovine Serine Albumin (BSA) in PBS, was added and the sample was nutated at 4°C

(18) HEY1-NCOA2 expression modulates chondrogenic differentiation and induces mesenchymal chondrosarcoma in miceJCI insightMay 22, 2023Tanaka M, Homme M, Teramura Y, Kumegawa K, Yamazaki Y, Yamashita K, Osato M, Maruyama R, Nakamura T.(Cell Signaling) antibodies. The antibody-bound protein/DNA complexes were immunoprecipitated using ChIP grade protein G magnetic beads (Cell Signaling). Immunoprecipitated DNA was then purified and subjected to secondary sonication to

(19) Tumor Cytokine-Induced Hepatic Gluconeogenesis Contributes to Cancer Cachexia: Insights from Full Body Single Nuclei SequencingbioRxiv : the preprint server for biologyMay 18, 2023Liu Y, Dantas E, Ferrer M, Liu Y, Comjean A, Davidson EE, Hu Y, Goncalves MD, Janowitz T, Perrimon N.(Cell Signaling, 2729) overnight at 4°C with rotation. 30 ul ChIP-Grade Protein G Magnetic Beads (Cell Signaling, 9006) were incubated with each immunoprecipitation for 2 hours at 4 °C with rotation. Beads were washed and

(20) Myc derived circRNA promotes triple-negative breast cancer progression via reprogramming fatty acid metabolismDiscover OncologyMay 12, 2023Shengting Wang, Yufang Wang, Yue Wang, Qian Li, Kaixuan Zeng, Xiaoming Li, Xinghua Fengand incubated at 4℃ for 4 h, followed by incubation with 50 µL ChIP-Grade Protein G Magnetic Beads (9006, Cell Signaling Technology)at 4 ℃ for 2 h. After washing, the beads were incubated with 150µL 1×ChIP

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